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The method is also well suited to examine the effectiveness of existing or novel interventions--such as new drug candidates - targeting the treatment of painful andor inflammatory conditions. This article will provide a detailed description on the use of microdialysis techniques for collecting interstitial fluid from experimentally inflamed skin lesion of human study subjects. Interstitial fluid samples are typically processed with aid of multiplex bead array immunoassays allowing assaying up to 100 analytes in samples as small in volume as 50 microliters. We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site.

Roy, being a fellow American, it was confusing to me at first as well. But the more I looked into it and talked to Aussies, the more it became efficient and understandable. Here, if no candidate wins a majority of the votes, they have a run off (or it goes to the Congress in the case of the President and VP). There, they count the votes. No one gets a majority of 1s, then they count the 2s. So no run off.

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